Identification of Distinct Potyviruses in Mixedly-Infected Sweetpotato by the Polymerase Chain Reaction with Degenerate Primers

Identification of potyviruses using the polymerase chain reaction with degenerate primers.

Local areas of conserved amino acid sequence in the replicase and coat proteins of potyviruses were used to select nucleotide sequences for use in the construction of sets of degenerate oligonucleotide primers for amplification of DNA fragments on potyvirus-specific templates in a combined assay of reverse transcription and the polymerase chain reaction (RT-PCR). Sequences selected for the cons...

Degenerate Primers Facilitate the Detection and Identification of Potyviruses From the Northwest Region of Iran

Background: Potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. Potyviruses can be transmitted through plant sap, seeds and many aphid species. Due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. Objectives: This study compared the efficiencies of two couples of primers in the...

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Characterization of superoxide dismutase Gram-positive bacteria by polymerase chain degenerate primers genes from reaction using

An internal fragment representing approximately 85% of sod genes from seven Gram-positive bacteria was amplified by using degenerate primers in a polymerase chain reaction assay. The DNA sequences of sod polymerase chain reaction products from Clostridium perfringens, Enterococcus faecalis, Enterococcus faecium, Lactococcus lactis, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus ...

Identification of Casuarina-Frankia strains by use of polymerase chain reaction (PCR) with arbitrary primers.

Free-living N2-fixing Frankia strains isolated from Casuarina sp. were investigated for genomic polymorphism. We used six 10-mer oligonucleotides as single arbitrary primers (AP) for the polymerase chain reaction (PCR) in order to amplify random DNA fragments in the genome of free-living Frankia strains. Agarose-gels of the amplified genomic DNA revealed that two of the six arbitrary primers sh...